将Flp的密码子优化版本Flpo插入到En1基因座1号外显子的ATG位置。通过Southern blot检测阳性ES细胞克隆,然后将它们注入囊胚,产生杂交小鼠。杂交小鼠与C57BL/6J雌性杂交。随后,通过Protamine::Cre小鼠去除Neomycin选择性载体。(来源:J:313825)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
--
1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
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