将Flp的密码子优化版本Flpo插入到En1基因座1号外显子的ATG位置。通过Southern blot检测阳性ES细胞克隆,然后将它们注入囊胚,产生杂交小鼠。杂交小鼠与C57BL/6J雌性杂交。随后,通过Protamine::Cre小鼠去除Neomycin选择性载体。(来源:J:313825)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count