Arhgap45^em1Ciphe

Introns 3 and 4 were targeted with two sgRNAs (targeting AAGAGGGGATCTCTGTCGTGGGG and CAGAGTGGAGTTCGGGAGTATGG) using CRISPR/Cas9 technology, resulting in the deletion of exon 4. Lack of peptide expression in T and B cells was experimentally confirmed. (J:282407)