通过CRISPR/Cas9技术,对 introns 3 和 4 用上了两个sgRNAs(针对 AAGAGGGGATCTCTGTCGTGGGG 和 CAGAGTGGAGTTCGGGAGTATGG),成功地删除了exon 4。在T和B细胞中表达肽的功能缺失,这一点在实验中得到了验证(来源:J:282407)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Endonuclease-mediated
基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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