CRISPR/Cas9技术产生了一个19bp的缺失(GGCCCGGGGCCAGACAGCA),导致了框移突变和早停翻译。 Western blot结果显示睾丸中没有检测到蛋白质。(来源:J:310334)
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CRISPR/Cas9 technology generated a 19 bp deletion (GGCCCGGGGCCAGACAGCA) in exon 2 leading to a frameshift mutation and premature translation termination. Western blot analysis confirmed absence of protein in testes. (J:310334)