Gt(ROSA)26Sor^{tm57(CAG-hop/EGFP)Hze}

The Rosa-CAG targeting vector CAG-FSF-LSL-Jaws-GFP-ER2-WPRE-bGHpA was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The hop/EGFP fusion protein (Halo57*K200R*W214F/EGFP or Jaws) is composed of a modified Halobacterium halobium (strain shark)-derived halorhodopsin (Halo57) fused in-frame via a 15 nucleotide linker to the N-terminus of enhanced green fluorescent protein (EGFP). The Halo57 sequence has K200R and W214F mutations to substantially boost photocurrents. An endoplasmic reticulum exporting sequence (ER2) from the Kir2.1 inward rectifier potassium channel gene (amino acids FCYENEV) at the C-terminal of EGFP improves membrane trafficking/prevents aggregation. It is a Cre and Flp dependent reporter with an optogenetic silencer function. (J:219930)