Targeting vector based on pKO scrambler V915 (Lexicon Genetics Inc., Woodland, TX) flanking a LoxP-pGK-Neo-LoxP selection cassette containing a BAC clone insertion containing intron 4, exon 5, and intron 5 of murine gene Pten (Gene ID 19211, NM 008960.2) with variant c.374A>G, p.Lys125Arg (K125R). (J:94077)