使用插入到内含子10的供体质粒进行CRISPR靶向,该质粒携带一个单拷贝的AcGFP报告基因,带有串联的HA标签、终止子、Strep/FLAG标签(SF)以及FRT3介导的 puromycin抗性元件。通过Flp介导的重组,选择性元件被去除。(来源:J:311810)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Applicable
Endonuclease-mediated
插入
--
1
8
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
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