Acss2^em1Reh

CRISPR/Cas9 technology generated acidic amino acid substitutions for basic amino acid residues, arginine and lysine (RK) to glutamate and aspartate (ED), in the putative nuclear localization signal KTRSGKIMRRVLRK. The mRNA levels in the homozygotes are reduced to approximately 50% of levels in wild-type mice while protein levels are reduced more significantly. (J:282290)