CRISPR/cas9 mediated recombination using 2 guide RNAs (sgRNA-1: ATCTTATGCTGGGTCCAGTAAGG, sgRNA-2: TTTATAACAAATGGCTTGATTGG) deleted a genomic region spanning exons 2 - 5. Immunostaining confirmed the absence of protein expression in testes and spermatozoa from homozygous mice. (J:311550)