Zfp469^em1Chms

CRISPR/Cas9 technology inserted an in-frame V5 tag and a premature stop codon at glycine 634 (p.Gly634) prior to the C2H2 zinc-finger domains. The V5 tag contains a spontaneously arising A>T mutation, resulting in the amino acid change Asn>Ile at position 5 in the V5 tag. RT-qPCR confirmed the presence of a truncated transcript. The stop codon at Gly634 corresponds to one of the brittle cornea syndrome mutations p.Gly677* in human. (J:311083)