通过TARGATT整合酶介导的 cassette exchange,包含有双向tetO(TRE)启动子驱动人类SHH cDNA表达和ZsGreen1荧光标记基因元件的靶向构建被插入到Gt(ROSA)26Sor的docking attP位点,通过phiC31整合酶实现。至少生成了三条克隆品系,其中2号和3号在报告基因表达上相似,2号品系被用于大部分研究。(来源:J:310473)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Not Specified
Targeted
插入
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--
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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