Dock8^tm2.1Ysfk

The targeting vector consists of mouse Dock8 gene encoding exon 3 flanked by two loxP sites, the PGK-neo cassette (Neo) flanked by two flippase recognition target (FRT) sites. After homologous recombination in ES cells and generation of chimeric male founders with correctly targetted ES cells, the PGK-neo cassette was excised by crossing with the CAG-FLPe transgenic mice. Cre-mediated excision of exon 3 results in the appearance of a premature stop codon in exon 4. (J:299945)