在exon 1上游序列和intron 8内,使用了一对sgRNAs,分别针对(2) CTGGTCTCAAGCTCACTAAG和(4) TTATATGCAATACCCAGCAT,目标是实现从sgRNA靶点2到4之间的基因片段删除,包括exons 1到8。这些ESC(胚胎干细胞)在构建这个等位基因时,还携带有Tg(CAG-EGFP,Acr-EGFP)2Osb双基因。(来源:J:306305)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb
Endonuclease-mediated
基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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