这种靶向突变在内源性exon 11的上游插入了lox511位点,下游则插入了一个由FRT引导的诺卡因抗性基因座,接着是带有氨基酸替换(L332A)的反向和修饰过的exon 11,以及另一个lox511位点。通过Flp酶介导的重组,去除了FRT标记的诺卡因抗性基因座。随后,Cre介导的重组移除了外源exon 11,恢复了突变的exon 11。来源:(J:290809)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion, Nucleotide substitutions
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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