Gt(ROSA)26Sor^em7Mvw

Using CRISPR-Cas9 and oligo-mediated homology directed repair, two heterologous attachment sites, Bxb1 attP-GT and Bxb1 attP-GA, were inserted into intron 1-2, providing cognate attachment and recombination sites for Bxb1 attB-GT and Bxb1 attB-GA, for unidirectional, single-copy donor transgenic integration mediated by Bxb1 Integrase. (J:188991, J:322700)