A targeting vector containing (in order from 5' to 3') encephalomyocarditis virus IRES, tandem dimer (td) Tomato, T2A self-cleaving peptide from Thosea asigna virus, Cre recombinase fused to the estrogen receptor ligand binding domain (ER), bovine growth hormone poly(A), and a frt-flanked neomycin resistance cassette was inserted in exon 8 downstream of the endogenous stop codon. The neomycin selection cassette was excised in male mice by crossing to FLP1 expressing female. (J:282429)