CRISPR/cas9基因编辑技术用于在第10外显子的第302位,从赖氨酸(K302E,AAA替换为GAA)修改为谷氨酸。此外,为了提高靶向效率,还包括了silent位点的改变,N303N(AAC变为AAT)。这种替换导致表达的是C57BL/6J小鼠正常表达的Ptprca(CD45.1或Ly5.1)基因,而非Ptprcb(CD45.2或Ly5.2)等位基因。参考文献:J:101977

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
核苷酸替换
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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