Ptprc^em6Lutzy

CRISPR/cas9 genome editing is used to alter a lysine to glutamic acid mutation at amino acid 302 (K302E ; AAA to GAA) in exon 10. Additionally, the silent nucleotide change N303N (AAC to AAT) was included for targeting efficiency. The substitution results in the expression of Ptprca (CD45.1 or Ly5.1) rather than the Ptprcb (CD45.2 or Ly5.2) allele normally expressed by C57BL/6J mice. (J:101977)