Gt(ROSA)26Sor^{tm2.1(CAG-HIST1H2BB/EGFP)Zjh}

The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a FRT-flanked STOP cassette, an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. The entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. Cre mediated recombination removed the loxP-flanked STOP cassette. This alle was generated by crossing mouse with parental Gt(ROSA)26Sortm2(CAG-HIST1H2BB/EGFP)Zjh to cre-deleter mouse. It is an flp reporter. (J:308660)