This insertion contains (in order) a CAGGS promoter, FRT-flanked neo cassette with 2 poly-adenylation sequences, floxed EGFP followed by 5 poly-adenylation sequences and a lacZ gene with a nuclear localization sequence. Parental Gt(ROSA)26Sortm5(CAG-EGFP,-lacZ)Dym was crossed to cre deleter mice to remove EGFP-STOP cassette. Nuclear-lacZ expression results from FLP- mediated recombination. (J:290856)