Trem2^em1Tcp

A G-to-A mutation was introduced to change arginine codon 47(CGC) to a histidine codon (CAC) (p.R47H) using an sgRNA (targeting ACTGGGGGAGACGCAAGGCC) and an ssODN template (CTGCAGGGCATGGCCGGCCAGTCCTTGAGGGTGTCATGTACTTATGACGCCTTGAAGCACTGGGGGAGACaCAAGGCCTGGTGTCGGCAGCTGGGTGAGGAGGGCCCATGCCAGCGTGTGGTGAGCACACACGGTGTGTGGCTGC) with CRISPR/Cas9 technology. The mutation has been indicated as an Alzheimer's Disease risk factor. (J:308299)