Tnf^em5Boui

The adenylate-uridylate-rich element (AU-rich element; ARE) and new regulatory element (NRE) in the 3' UTR were targeted with gRNAs (targeting GTGCAAATATAAATAGAGGG, TGCTTATGAATGTATTTATT, TTGTCTTAATAACGCTGATT and ATTTCTCTCAATGACCCGTA) using CRISPR/Cas9 technology, resulting in a 71 bp and a 76 bp deletion (TCTATTTATATTTGCACTTATTATTTATTATTTATTTATTATTTATTTATTTGCTTATGAATGTATTTATT and GCTGATTTGGTGACCAGGCTGTCGCTACATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATCGCCCTA). (J:306876)