CRISPR/Cas9技术针对2号外显子两端进行编辑,删除了大约5KB的基因区域,包括大部分编码序列。 Western blot和RT-qPCR结果显示没有检测到蛋白和转录本的存在。(来源:J:306854)
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CRISPR/Cas9 technology targeting the two ends of exon 2 deleted exon 2 (approximately 5 kb of the gene, deleting the majority of the coding sequence). Western blot and RT-qPCR confirmed absence of protein and transcript. (J:306854)