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CRISPR/Cas9 endonuclease-mediated genome editing is used to create a F352V missense mutation (TTT to GTT). Guide RNAs (TTCTCAGATTCAGTAAAATC and ATTTTACTGAATCTGAGAAG) were selected to target position 352 of exon 2. The mutation corresponds to a human mutation associated with decreased susceptibility to late-onset Alzheimer's disease. (J:101977)