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CRISPR/Cas9 endonuclease-mediated genome editing is used to introduce encoding a S370C missense mutation (TCC to TGC). Additionally, the following silent nucleotide change was included for targeting efficiency (L370L [CTC to CTT]. Guide RNAs (GACTTTTTTGCTCTCTCCTT and AAAGCTCAAGGTTGGTCCGA) were selected to target position 370 of exon 2. The mutation that corresponds to the human C370 codon associated with late-onset Alzheimer's disease. (J:101977)