Cct6b^em1Jsha

CRISPR/Cas9 technology generated a 5bp deletion (c.373_377delAACTG) in exon 4, with the glutamate at position 125 as the first amino acid changed, shifting the reading frame, replacing it for a glycine and terminating at position Ter22 (p.Glu125GlyfsTer22). Western blotting and qPCR confirmed the absence of protein and reduced levels of mRNA in the testis, respectively. (J:307593)