Exon 2通过CRISPR/Cas9技术用sgRNA进行了靶向,产生了49bp的缺失。 Western blot和免疫组化实验确认了睾丸中这个等位基因没有肽表达(来源:J:307539)
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Exon 2 was targeted with an sgRNA using CRISPR/Cas9 technology, resulting in a 49 bp deletion. Western blot and immunohistochemistry experiments confirmed the lack of peptide expression from this allele in testes. (J:307539)