Ddi1^em1Qsh

The single-exon gene was targeted with an sgRNA (targeting GCTGTTCCATGTAAACGATC) using CRISPR/Cas9 technology, resulting in a 26 bp deletion (c.120-145del, p.P41Hfs*8). Western blot and immunohistochemistry experiments confirmed the absence of peptide expression in testes. (J:307592)