The targeting vector contains a T2A-tdTomato-FRT-Neo-FRT cassette, which replaces the stop codon just before the 3' untranslated region. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:286603)
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The targeting vector contains a T2A-tdTomato-FRT-Neo-FRT cassette, which replaces the stop codon just before the 3' untranslated region. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:286603)