Gt(ROSA)26Sor^{tm1.1(CAG-CHRM3*/mCherry)Stak}

The targeting construct consisting of a CAG promoter, a loxp, 2xpA, FRT-flanked Pgk-neo selection cassette, STOP cassette, loxp, followed by a hM3Dq/mCherry fusion protein, pA signal all inserted into the Gt(ROSA)26Sor locus was used to generate the mouse line by homologous recombination in the C57BL/6N ES cells. The pgk-Neo cassette was removed with FLP66. CAG promoter drives expression of hM3Dq-mCherry only after Cre-mediated excision of the floxed stopper element. (J:294111)