这种突变是通过插入编码增强绿色荧光蛋白(EGFP)的序列,替换掉317号和18号外显子的部分片段,后面跟着人类生长激素的poly(A)序列和抗氨苄青霉素抗性基因。在4周大的纯合小鼠大脑中,通过RT-PCR和 Western blot 分析,没有检测到EGFP的表达。Plexin-B3的内源性mRNA和蛋白质表达也均无法检测到。(来源:J:307447)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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