Plxnb3

The mutation is a deletion of exons 317 and part of exon 18 by replacing them with a sequence encoding for enhanced green fluorescent protein (EGFP) followed by a human growth hormone polyadenylation sequence and a neomycin resistance cassette. EGFP expression was not detected in the mutant animals. Endogenous Plexin-B3 mRNA and Plexin-B3 protein expression was undetectable via RT-PCR and Western blot analysis respectively, in brains of 4-week-old homozygous mice. (J:307447)