在小鼠胚胎干细胞中,通过同源重组技术,将一个改良型Cre重组酶基因和带有FRT切割位点的诺卡霉素抗性基因,通过内源性核糖体进入序列(IRES)连接到Npvf基因的3'非翻译区末端,这个位置紧邻终止密码子。随后,通过FLPe剔除小鼠与之杂交,以去除FRT标记的诺卡霉素筛选元件。(来源:J:300737)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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