通过在ES细胞中通过同源重组靶向,将包含起始密码子的exon 3(ENSMUSE00001275521)用loxP序列包裹,后面跟着由Frt序列包裹的Pgk-neo cassette。随后通过与Rosa-Flp小鼠杂交移除Pgk-neo cassette(J:282136)。
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count