一种靶向质粒,它包含猪teschoviral 2A切割序列,连接到Cre重组酶,以及替换Calcr基因终止密码子的Frt座标记的诺卡因抗性基因,用于胚胎干细胞定向。通过将这些质粒导入细菌人工染色体(RP24-193M22),产生了杂交动物。随后,这些杂合子与Flp剔除小鼠杂交,以去除诺卡因选择标记片段。(来源:J:260503)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
1
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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