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中文
构建的融合蛋白是rtTA*M2的tetracycline响应域（来自Clontech的pTet-on Advanced）和SDKid-1的KRAB-AB沉默域（Clontech的pTet-tTS），命名为rtTS（tetracycline-controlled trans-silencer）。SA70b的pROSA-CAGGS-attP50/B53-hygro-NLSLacZ载体来自ARTEMIS Pharmaceuticals。从SA70b中去除CAGGS启动子和内含子片段，并插入到修改过的pROSA26质粒中，形成了pROSA26CAG-PAS载体。rtTS的cDNA被克隆进pBigT，再转接到pROSA26CAG-PAS，最终得到了定向到Rosa26的CAG-LSL-rtTS载体（J:255811）。
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
