E2f3^tm1.1Gev

A vector containing 15 kb of the endogenous genomic sequence. The tetracycline response element (TRE) element was isolated from pTre2 (Clontech) and then cloned 500bp upstream of the translation start site along with a loxP flanked neomycin resistance cassette. No endogenous gene sequences were removed or altered at the insertion site, although silent mutations were incorporated into the first exons of E2f3a and E2f3b. Finally, a DTA cassette was added to this vector to allow negative selection in mouse embryonic stem cells. Cre-mediated recombination removed the conditional neomycin resistance cassette. (J:255811)