Hoxa13^{tm1.1(cre/ERT2)Mtor}

The targeting vector containing Cre-ERT2-FRT-Neo-FRT-LoxP cassette inserted into exon 1 just downstream of the endogenous ATG site of Hoxa13 gene, replacing 1864 base pairs of the coding region spanning exons 1 and 2 was generated from a C57BL/6 BAC (bacterial artificial chromosome) clone (RPCI23:33N14) using homologous recombination. C57BL/6NTac 129S6/SvEvTac embryonic stem (ES) cells (Taconic) were electroporated with the targeting construct to generate chimera. After germline transmission, the Neo resistance cassette was eliminated by breeding with a Flpe deleter line. (J:301894)