Ip6k1^em1Seyk

Exon 2, containing the ATG start codon, was targeted with two sgRNAs (targeting TTTGTCAAACCATGGAAGTGGGG and CCGCCCACCTGATGGATGAAGGG) using CRISPR/Cas9 technology, resulting in a 73 bp deletion. Absence of peptide expression from this allele was confirmed by immunoblot experiments with brain extracts. (J:306957)