使用了FlpO-lox2722-PGK-Neo-lox2722的 cassette,通过同源重组在ES细胞中替换小鼠Atoh1基因的编码序列,保持了转录起始点不变。随后通过和表达Cre的鼠杂交去除PGK-neo cassette(来源:J:268187)。
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count