设计的质粒含有所需的导向RNA序列,分别为AAATCTGATGATCTCAGTGA和TAAATCTGATGATCTCAGTG,它们的目标是将ACC转换为TCA,导致人类CR1基因(在第29外显子的第1610位,标记为T1610S)上的一个丝氨酸替换为苏氨酸。这些质粒原本被插入了小鼠的Cr2位点(来源:J:101977)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6(SJL)-Cr2tm1(CR2,CR1)How Apoetm1.1(APOE*4)Adiuj Trem2em1Adiuj/J
Endonuclease-mediated
Insertion, Nucleotide substitutions
--
1
5
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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