这个等位基因是通过CRISPR-Cas9介导的同源重组,在R1胚胎干细胞中,将cre/ERT2启动子插入到了小鼠Clu基因的起始翻译位点上创建的。随后,通过和表达Flp重组酶的 mice杂交,去除了含neomycin的 cassette。(来源:J:283362)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Endonuclease-mediated
插入
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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