Il11^em2Tsak

Exon 3 was targeted with sgRNAs using CRISPR/Cas9 technology by micro-injecting a plasmid coding for two gRNAs and a Fok1-dCas9 fusion gene (restriction enzyme FokI and nuclease-deficient Cas9) into fertilized oocyte pronuclei. In founder line 11, this resulted in a 19 bp deletion and a point mutation (or a 20 bp deletion and 1 bp insertion or duplication). (J:273448)