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Exon 3 was targeted with sgRNAs using CRISPR/Cas9 technology by micro-injecting a plasmid coding for two gRNAs and a Fok1-dCas9 fusion gene (restriction enzyme FokI and nuclease-deficient Cas9) into fertilized oocyte pronuclei. In founder line 5, this resulted in an 82 bp deletion. (J:273448)