The targeting construct replaced about 500 bp of the coding sequence in exon 1 with a membrane-localized tdTomato reporter gene (mTomato), a T2A sequence, nuclear-localized GFP (H2B:GFP), and polyadenylation sequence (bGH PolyA). Cre-mediated recombination removed the floxed neomycin selection cassette introduced downstream of the inserted sequence. (J:243419)