TALEN介导的重组在exon 1中插入了splice接受器位点、Venus报告基因以及pgk-puromycin抗性元件。RT-PCR和 Western blot结果显示,纯合小鼠骨髓中未检测到表达(来源:J:301766)
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TALEN mediated recombination inserted a splice acceptor site, Venus reporter gene, and pgk-puromycin resistance cassette in exon 1. RT-PCR and western blot analysis confirmed the absence of expression in bone marrow from homozygous mice. (J:301766)