通过同源重组,将包含所有1号外显子的基因区域替换为pgk-neo载体。 Western blot结果显示,纯合小鼠中没有检测到蛋白表达。(来源:J:298039)
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A genomic region including all of exon 1 was replaced with a pgk-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in homozygous mice. (J:298039)