Tmem175^em1Dren

CRISPR/Cas9 technology using sgRNAs targeting exon 3 (ttctaatcgtaactgtggcctgg) and exon 4 (tacacttgccttgctcaacctgg) deleted part of exon 3 and 4 (20 nucleotides of exon 3, 2.2 kb intron, and 47 neucleotides of exon 4), starting at the codon that encodes A88 in the second transmembrane domain, resulting in an out-of-frame truncation. (J:304120)