Cttnbp2^em2Yph

CRISPR/Cas9 technology changed two nucleotides (T to A produces the stop codon, while a C to T change seven bp upstream affects the XmaI site) to alter the coding sequences of arginine to a stop codon at residue 533 (R533*) in exon 4. This mutation corresponds to the autism spectrum disorder-linked R536* mutation in patients. (J:300704)