Gabra5^tm1.1Krmr

The allele was designed to introduce a glutamic acid-to-cysteine mutation at codon 125 (E152C counting from the start codon; or E125C in the mature peptide sequence) into exon 5 of the gene. The targeting vector also includes a loxP-flanked neomycin selection cassette upstream of exon 5, as well as a C-to-T silent mutation in the exon 5 DNA sequence (to create a HindIII site upstream of the mutation for genotyping). Cre-mediated recombination removed the floxed neo cassette. (J:300549)