一段1.15千碱基的区域,包括6和7号外显子,通过同源重组被pgk-neo cassette替换了。RT-PCR和 Western blot的结果证实了纯合小鼠大脑区域中没有mRNA和蛋白质的存在。(来源:J:288931)
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A 1.15 kb region including exons 6 and 7 was replaced with a pgk-neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of mRNA and protein in forebrain regions from homozygous mice. (J:288931)