Prss59^em1Fcw

CRISPR/Cas9 technology deleted 330 bp which includes exon 4 resulting in a frameshift mutation. Sequencing of the RT-PCR amplicon confirmed deletion of exon 4 and a splicing of exon 3 to exon 5. The frameshift mutation occurred from valine 14 to the last amino acid resulting in a new stop codon at the position of the 40th codon. (J:303285)