CRISPR/Cas9技术通过两个sgRNA(sgRNA-1: TTGGTCCGGACGTCCTCCAG, sgRNA-2: TGGTAATAACCGAGACGAAG)对基因组进行编辑,产生了11,771bp的exon 4至exon 11的缺失。成人睾丸的western blot分析证实了相应蛋白的缺失。(来源:J:303296)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count